In huge breakthrough, scientists regenerate human heart tissue from skin cells

Anyone who has undergone a heart transplant, or has friends/relatives who went under the knife, can tell you about the mind-numbing wait for a heart that is an exact match.

If a recent scientific breakthrough is anything to go by, then the day that heart transplant patients need no longer wait for a match is finally drawing nearer.

Researchers from Massachusetts General Hospital have successfully grown functional heart tissue from stem cells created from skin cells. Their paper has been published in the journal Circulation Research.

The team's technique potentially allows heart tissue to be built with the patient's own cellular material, which reduces the need for an exact donor match, and also vastly lowers the chance of immunorejection.

It's not possible to simply grow an entire heart from cells. Organs require a scaffold to give the cells a shape. In the normal course of things, this scaffold, known as an extracellular matrix, is created from proteins secreted by the cells.

Rather than grow these extracellular matrices, which would take time, the team used 73 donor hearts from the New England Organ Bank. These hearts had been determined unsuitable for transplantation.

To prepare the hearts, the team stripped them down to the extracellular matrix with a detergent solution. This removes all the living cells and leaves behind a neutral scaffold for the new cellular material.

To seed the matrices with cells, the team used a newer method that uses messenger RNA to revert the skin cells to stem cells, a more efficient technique than the older genetic manipulation. These pluripotent stem cells were then induced to grow into cardiac muscle cells.

These muscles cells were then introduced into the extracellular matrix. Within days, they grew into contracting muscle tissue. Finally, the growing heart was placed in a bioreactor with a nutrient solution, and stressors that reproduced the conditions under which a living heart operates.

After 14 days, the team found dense regions of immature cardiac muscle tissue that contracted normally under electrical stimulation.

Courtesy: cnet.com